SCF/GGA Troubleshooting

High SCF, Low GGA? please check the following:

• Is the GGA fresh and of high quality? Always ensure that the GGA solution has not expired and has not been contaminated. Remember, exposure to air can biologically contaminate the GGA solution.
• Has your labware been properly cleaned? Always properly clean and store your bottles, pipettes, tubing, air stones, etc. Sterilization of air stones and tubing is not recommended.
• Has your technique changed? Make sure that your technique is proper and consistent, paying special attention to your pipetting technique.
• Have you checked the water quality? The DI/Distilled water pH should be between 6.5 and 7.5 with a conductivity of less than 1.0 micromhos. The BOD water pH should be 7.2+-0.2 with a conductivity of 125 – 160 micromhos.
• Finally, have you reviewed and are properly following the current PolySeed or PolySeed NX application procedures? A copy of these procedures is provided with each bottle of capsules or liquid and should be reviewed regularly for changes. These procedures can also be downloaded from our website.Next, after the above has been addressed and problems still exist, do the following:Increase PolySeed or PolySeed NX rehydration dilution water by 50-250 mls. This process is approximately linear so if you need to reduce the SCF 20% you should increase the dilution water by 20%.After this adjustment and your SCF falls into range but you are still having problems with low GGA, increase the amount of seed in your GGA’s and/or samples by 1-2 mls which will increase the GGA.

• Is the GGA fresh and of high quality? Always ensure that the GGA solution has not expired and has not been contaminated. Remember, exposure to air can biologically contaminate the GGA solution.
• Has your labware been properly cleaned? Always properly clean and store your bottles, pipettes, tubing, air stones, etc. Sterilization of air stones and tubing is not recommended.
• Has your technique changed? Make sure that your technique is proper and consistent, paying special attention to your pipetting technique.
• Have you checked the water quality? The DI/Distilled water pH should be between 6.5 and 7.5 with a conductivity of less than 1.0 micromhos. The BOD water pH should be 7.2+-0.2 with a conductivity of 125 – 160 micromhos.
• Finally, have you reviewed and are properly following the current PolySeed or PolySeed NX application procedures? A copy of these procedures is provided with each bottle of capsules or liquid and should be reviewed regularly for changes. These procedures can also be downloaded from our website.Next, after the above has been addressed and problems still exist, do the following:Decrease PolySeed or PolySeed NX rehydration dilution water by 50-250 mls. This process is approximately linear so if you need to increase the SCF 20% you should decrease the dilution water by 20%.After this adjustment and your SCF falls into range but you are still having problems with high GGA, decrease the amount of seed in your GGA’s and/or samples by 1-2 mls which will lower the GGA.

Low SCF, Low GGA? please check the following:

• Is the GGA fresh and of high quality? Always ensure that the GGA solution has not expired and has not been contaminated. Remember, exposure to air can biologically contaminate the GGA solution.
• Has your labware been properly cleaned? Always properly clean and store your bottles, pipettes, tubing, air stones, etc. Sterilization of air stones and tubing is not recommended.
• Has your technique changed? Make sure that your technique is proper and consistent, paying special attention to your pipetting technique.
• Have you checked the water quality? The DI/Distilled water pH should be between 6.5 and 7.5 with a conductivity of less than 1.0 micromhos. The BOD water pH should be 7.2+-0.2 with a conductivity of 125 – 160 micromhos.
• Finally, have you reviewed and are properly following the current PolySeed or PolySeed NX application procedures? A copy of these procedures is provided with each bottle of capsules or liquid and should be reviewed regularly for changes. These procedures can also be downloaded from our website.Next, after the above has been addressed and problems still exist, do the following:Decrease PolySeed or PolySeed NX rehydration dilution water by 50-250 mls. This process is approximately linear so if you need to increase the SCF 20% you should decrease the dilution water by 20%.After this adjustment and your SCF falls into range but you are still having problems with low GGA, increase the amount of seed in your GGA’s and/or samples by 1-2 mls which will increase the GGA.

Good SCF, High GGA? please check the following:

• Is the GGA fresh and of high quality? Always ensure that the GGA solution has not expired and has not been contaminated. Remember, exposure to air can biologically contaminate the GGA solution.
• Has your labware been properly cleaned? Always properly clean and store your bottles, pipettes, tubing, air stones, etc. Sterilization of air stones and tubing is not recommended.
• Has your technique changed? Make sure that your technique is proper and consistent, paying special attention to your pipetting technique.
• Have you checked the water quality? The DI/Distilled water pH should be between 6.5 and 7.5 with a conductivity of less than 1.0 micromhos. The BOD water pH should be 7.2+-0.2 with a conductivity of 125 – 160 micromhos.
• Finally, have you reviewed and are properly following the current PolySeed or PolySeed NX application procedures? A copy of these procedures is provided with each bottle of capsules or liquid and should be reviewed regularly for changes. These procedures can also be downloaded from our website.Next, after the above has been addressed and problems still exist, do the following:If your SCF falls into range but you are having problems with high GGA, decrease the amount of seed in your GGA’s and/or samples by 1-2mls which will lower the GGA.

Good SCF, Low GGA? please check the following:

• Is the GGA fresh and of high quality? Always ensure that the GGA solution has not expired and has not been contaminated. Remember, exposure to air can biologically contaminate the GGA solution.
• Has your labware been properly cleaned? Always properly clean and store your bottles, pipettes, tubing, air stones, etc. Sterilization of air stones and tubing is not recommended.
• Has your technique changed? Make sure that your technique is proper and consistent, paying special attention to your pipetting technique.
• Have you checked the water quality? The DI/Distilled water pH should be between 6.5 and 7.5 with a conductivity of less than 1.0 micromhos. The BOD water pH should be 7.2+-0.2 with a conductivity of 125 – 160 micromhos.
• Finally, have you reviewed and are properly following the current PolySeed or PolySeed NX application procedures? A copy of these procedures is provided with each bottle of capsules or liquid and should be reviewed regularly for changes. These procedures can also be downloaded from our website.Next, after the above has been addressed and problems still exist, do the following:If your SCF falls into range but you are having problems with low GGA, increase the amount of seed in your GGA’s and/or samples by 1-2mls which will increase the GGA.

High SCF, Good GGA? please check the following:

• Is the GGA fresh and of high quality? Always ensure that the GGA solution has not expired and has not been contaminated. Remember, exposure to air can biologically contaminate the GGA solution.
• Has your labware been properly cleaned? Always properly clean and store your bottles, pipettes, tubing, air stones, etc. Sterilization of air stones and tubing is not recommended.
• Has your technique changed? Make sure that your technique is proper and consistent, paying special attention to your pipetting technique.
• Have you checked the water quality? The DI/Distilled water pH should be between 6.5 and 7.5 with a conductivity of less than 1.0 micromhos. The BOD water pH should be 7.2+-0.2 with a conductivity of 125 – 160 micromhos.
• Finally, have you reviewed and are properly following the current PolySeed or PolySeed NX application procedures? A copy of these procedures is provided with each bottle of capsules or liquid and should be reviewed regularly for changes. These procedures can also be downloaded from our website.Next, after the above has been addressed and problems still exist, do the following:If your GGA falls into range but you are having problems with high SCF, increase the PolySeed or PolySeed NX rehydration dilution water by 50-250 mls. This process is approximately linear so if you need to decrease the SCF by 20% you should increase the dilution water by 20%.

Low SCF, Good GGA? please check the following:

• Is the GGA fresh and of high quality? Always ensure that the GGA solution has not expired and has not been contaminated. Remember, exposure to air can biologically contaminate the GGA solution.
• Has your labware been properly cleaned? Always properly clean and store your bottles, pipettes, tubing, air stones, etc. Sterilization of air stones and tubing is not recommended.
• Has your technique changed? Make sure that your technique is proper and consistent, paying special attention to your pipetting technique.
• Have you checked the water quality? The DI/Distilled water pH should be between 6.5 and 7.5 with a conductivity of less than 1.0 micromhos. The BOD water pH should be 7.2+-0.2 with a conductivity of 125 – 160 micromhos.
• Finally, have you reviewed and are properly following the current PolySeed or PolySeed NX application procedures? A copy of these procedures is provided with each bottle of capsules or liquid and should be reviewed regularly for changes. These procedures can also be downloaded from our website.Next, after the above has been addressed and problems still exist, do the following:If your GGA falls into range but you are having problems with low SCF, decrease the PolySeed or PolySeed NX rehydration dilution water by 50-250 mls. This process is approximately linear so if you need to increase the SCF by 20% you should decrease the dilution water by 20%.